Markerless cell sorting

Current techniques for sorting cells often require the use of optical (e.g. fluorescent) dyes, which are bound to specific structures or compounds within the cell, and used to differentiate cell-types from each other. However, some of these compounds can be toxic and limit the usefulness of cells, once sorted. In this project, we wish to develop improved markerless methods of cell-sorting during the process of cell culture. 

We have been working with local companies who are involved in muscle cell culture (e.g. for laboratory-grown ‘meat’) and cell-sorting (i.e. cytometry). In this project, with industry guidance, we will develop and test new methods for high-speed markerless cell sorting and apply these techniques in devices for the use of sorting cells from living populations. Applications include processing cell lines for laboratory-grown meat, and isolating healthy cardiac myocytes digested from samples of living heart tissue. 

This project will involve microfluidic design, optical design and implementation, high speed signal/image acquisition, and the development of analytical techniques (possibly including machine learning) for classifying cells in fluid using such signals. The project may also be suitable for a PhD project. 

The project would suit a student with a background or interest in biomedical engineering, physics, mechatronics, or similar fields.

This project is eligible for funding but is subject to eligibility criteria & funding availability.

Page expires: 20 December 2025